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Goat Anti-CP110 Antibody (EB11290)

Related Products: EBP11290 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB11290 Goat Anti-CP110 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB11290 1
EB11290 (1µg/ml) staining of Human Testis lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: centrosomal protein CP110, centrosomal protein of 110 kDa, Cep110, DKFZp781G1416, KIAA0419, OTTHUMP00000162162, CP110
Official Symbol: CP110
Accession Number(s): NP_001185951.1; NP_055526.3
Human GeneID(s): 9738
Important Comments: This antibody is expected to recognize both reported isoforms (NP_001185951.1; NP_055526.3).

Immunogen

Peptide with sequence CEKSLARIQEAS, from the N Terminus of the protein sequence according to NP_001185951.1; NP_055526.3.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 110kDa band observed in Human Testis lysates (calculated MW of 113kDa according to NP_001185951.1). Recommended concentration: 0.3-1µg/ml. Additional bands of unknown identity were also consistently observed at 65kDa and 37kDa. These bands were successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/specific breakdown fragments been reported?

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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