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Goat Anti-S100A8 Antibody (EB11513)

Related Products: EBP11513 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB11513 Goat Anti-S100A8 Antibody Pep-ELISA, IF, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]), fax (888-841-9041), phone (888-320-4628). Please include the following information with your order:

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In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day.

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB11513 1
EB11513 (5µg/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB11513 2
EB11513 Immunofluorescence analysis of paraformaldehyde fixed THP-1 cells immobilized on ShifixTM coverslip, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear and cytoplasmic/extracellular staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Click here to download PDF datasheet.

Target Protein

Principal Names: 60B8AG, CAGA, calgranulin A, calgranulin-A, calprotectin L1L subunit, CFAG, CGLA, CP-10, cystic fibrosis antigen, L1Ag, leukocyte L1 complex light chain, MA387, MIF, migration inhibitory factor-related protein 8, MRP8, MRP-8, NIF, OTTHUMP00000015329, P8, protein S100-A8, S100 calcium binding protein A8, S100 calcium-binding protein A8 (calgranulin A), S100A8, urinary stone protein band A
Official Symbol: S100A8
Accession Number(s): NP_002955.2
Human GeneID(s): 6279

Immunogen

Peptide with sequence C-KGADVWFKELDINT, from the internal region of the protein sequence according to NP_002955.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
IHC: Paraffin embedded Human Spleen. Recommended concentration: 5µg/ml.

Immunofluorescence: Strong expression of the protein seen in the nuclei and cytoplasmic/extracellular of THP-1 cells. Recommended concentration: 10µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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