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Goat Anti-PRMT2 Antibody (EB11558)

Related Products: EBP11558 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB11558 Goat Anti-PRMT2 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human, Mouse, Rat, Pig $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB11558 1
EB11558 (2µg/ml) staining of HeLa lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB11558 2
EB11558 (2µg/ml) staining of Mouse (A) and Rat (B) Brain lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB11558 3
EB11558 (2µg/ml) staining of Pig Brain lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: histone-arginine N-methyltransferase PRMT2, HMT1 (hnRNP methyltransferase, S. cerevisiae)-like 1, HMT1 hnRNP methyltransferase-like 1, HRMT1L1, MGC111373, OTTHUMP00000046525, OTTHUMP00000046526, OTTHUMP00000115881, OTTHUMP00000115882, OTTHUMP00000115884, OTTHUMP00000207831, PRMT2, PRMT2 alpha, PRMT2 beta, PRMT2 gamma, protein arginine methyltransferase 2, protein arginine N-methyltransferase 2
Official Symbol: PRMT2
Accession Number(s): NP_001526.2; NP_001229793.1; NP_001229794.1; NP_001229795.1
Human GeneID(s): 3275
Non-Human GeneID(s): 15468 (mouse), 499420 (rat)
Important Comments: This antibody is expected to recognize all reported isoforms (NP_001526.2; NP_001229793.1; NP_001229794.1; NP_001229795.1). Reported variants represent identical protein: NP_001526.2, NP_996845.1

Immunogen

Peptide with sequence C-DQPRTTKYHSVILQ, from the internal region of the protein sequence according to NP_001526.2; NP_001229793.1; NP_001229794.1; NP_001229795.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx 37kDa band observed in lysates of cell line HeLa and in rodent Brain lysates (calculated MW of 37.2kDa according to NP_001229793.1). Approx 35kDa band observed in Pig Brain lysates (calculated MW of 38.7kDa according to XP_005658963.1).Recommended concentration: 1-3µg/ml.

Species Reactivity

Tested: Human, Mouse, Rat, Pig
Expected from sequence similarity: Human, Mouse, Rat, Pig, Cow

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