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Goat Anti-EEA1 (aa821-835) Antibody (EB11689)

Related Products: EBP11689 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB11689 Goat Anti-EEA1 (aa821-835) Antibody Pep-ELISA, WB, IF Yes 100µg specific antibody in 200µl Human, Mouse $411.00 Elite

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Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]), fax (888-841-9041), phone (888-320-4628). Please include the following information with your order:

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In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day.

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB11689 1
EB11689 (0.3µg/ml) staining of HEK293 lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB11689 2
EB11689 Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (5ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing vesicle/cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (5ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Click here to download PDF datasheet.

Target Protein

Principal Names: early endosome antigen 1, early endosome antigen 1, 162kD, early endosome-associated protein, EEA1, endosome-associated protein p162, MST105, MSTP105, ZFYVE2, zinc finger FYVE domain-containing protein 2
Official Symbol: EEA1
Accession Number(s): NP_003557.2
Human GeneID(s): 8411

Immunogen

Peptide with sequence C-QETKIQHEELNNRIQ, from the internal region of the protein sequence according to NP_003557.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 200kDa band observed in lysates of cell line HEK293 (calculated MW of 162kDa according to NP_003557.2). This molecular weight is routinely observed by other sources. Recommended concentration: 0.3-1µg/ml. Primary incubatiion 1 hour at room temperature.

Immunofluorescence :Strong expression of the protein seen in the vesicles/cytoplasm of NIH3T3 cells. Recommended concentration: 5µg/ml.

Species Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Human

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