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Goat Anti-EAAT1 / GLAST1 Antibody (EB11916)
| Code | Name | Applications | Availability | Size | Tested species | Price | Grade | |
|---|---|---|---|---|---|---|---|---|
| EB11916 | Goat Anti-EAAT1 / GLAST1 Antibody | Pep-ELISA, WB |  |
100µg specific antibody in 200µl | Human | $423.00 |  |
|
Ordering Information for United States of America (change)Order direct from Everest
$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories. |
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EB11916 (2µg/ml) staining of Human Hippocampus lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Target Protein
Principal Names: SLC1A3, solute carrier family 1 (glial high affinity glutamate transporter), member 3, EA6, EAAT1, FLJ25094, GLAST, GLAST1, GLAST-1, OTTHUMP00000116002, excitatory amino acid transporter 1, sodium-dependent glutamate/aspartate transporter 1, solute carrier family 1 member 3Official Symbol: SLC1A3
Accession Number(s): NP_004163.3; NP_001160167.1
Human GeneID(s): 6507
Important Comments: This antibody is expected to recognize isoform 1 (NP_004163.3) and isoform 2 (NP_001160167.1).
Immunogen
Peptide with sequence C-DNETEKPIDSETK, from the C Terminus of the protein sequence according to NP_004163.3; NP_001160167.1.
Please note the peptide is available for sale.
Purification and Storage
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Tested
Peptide ELISA: antibody detection limit dilution 1:8000.Western blot: Approx 60kDa band observed in Human Brain (Hippocampus) lysates (calculated MW of 59.6kDa according to NP_004163.3). Recommended concentration: 1-3µg/ml.
Species Reactivity
Tested: HumanExpected from sequence similarity: Human