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Goat Anti-tropomyosin 4 / TPM4 Antibody (EB12417)

Related Products: EBP12417 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB12417 Goat Anti-tropomyosin 4 / TPM4 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human, Mouse, Pig $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB12417 1
EB12417 (0.03µg/ml) staining of Human Platelets lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB12417 2
EB12417 (0.1µg/ml) staining of NIH3T3 lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB12417 3
EB12417 (0.5µg/ml) staining of Pig Heart lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB12417 4
EB12417 (2.5µg/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB12417 5
EB12417 (2.5µg/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: TPM4, tropomyosin 4, TM30p1, tropomyosin alpha-4 chain, tropomyosin-4
Official Symbol: TPM4
Accession Number(s): NP_001138632.1; NP_003281.1
Human GeneID(s): 7171
Non-Human GeneID(s): 326618 (mouse), 24852 (rat)
Important Comments: This antibody is expected to recognize both reported isoforms (NP_001138632.1; NP_003281.1).

Immunogen

Peptide with sequence C-ENVGLHQTLDQ, from the C Terminus of the protein sequence according to NP_001138632.1; NP_003281.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 32kDa band observed in Human Platelets, Pig Heart and in NIH3T3 lysates (calculated MW of 32.7kDa according to NP_001138632.1). Recommended concentration: 0.1-0.3µg/ml. Primary incubation was 1 hour.
IHC: Paraffin embedded Human Placenta and Prostate. Recommended concentration: 2.5µg/ml.

Species Reactivity

Tested: Human, Mouse, Pig
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

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