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Goat Anti-CAPG (aa205-217) Antibody (EB12422)

Related Products: EBP12421 - Immunizing Peptide, EBP12422 - Immunizing Peptide, Goat Anti-CAPG Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB12422 Goat Anti-CAPG (aa205-217) Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human, Mouse, Rat, Pig $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB12422 1
EB12422 (0.3µg/ml) staining of U937 (A) and Human Spleen (B) lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB12422 2
Anti-CAPG (0.3µg/ml) staining of Periheral Blood Lymphocytes (EB12421 lane A), EB12422 lane B) lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB12422 3
EB12422 (0.1µg/ml) staining of Mouse (A) and Rat Spleen (B) lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB12422 4
EB12422 (0.3µg/ml) staining of Pig Spleen lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: CAPG, capping protein (actin filament), gelsolin-like, AFCP, MCP, actin regulatory protein CAP-G, actin-regulatory protein CAP-G, gelsolin-like capping protein, macrophage capping protein, macrophage-capping protein
Official Symbol: CAPG
Accession Number(s): NP_001738.2
Human GeneID(s): 822
Important Comments: This antibody is expected to recognize isoform 1 (NP_001738.2) only. Reported variants represent identical protein: NP_001243068.1, NP_001738.2.

Immunogen

Peptide with sequence C-KAQVEIVTDGEEP, from the internal region of the protein sequence according to NP_001738.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 38kDa band observed in lysates of cell line U937 and of Human, Mouse and Rat Spleen (calculated MW of 38.5kDa according to NP_001738.2). In Peripheral Blood Lymphocytes, the observed band runs as ~40kDa. Recommended concentration: 0.1-0.3µg/ml.

Species Reactivity

Tested: Human, Mouse, Rat, Pig
Expected from sequence similarity: Human, Dog, Pig

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