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Goat Anti-RSPO3 Antibody (EB13098)

Related Products: EBP10509 - Immunizing Peptide, EBP13098 - Immunizing Peptide, Goat Anti-RSPO3 Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB13098 Goat Anti-RSPO3 Antibody Pep-ELISA, WB, IHC Allow up to 10 days for delivery 100µg specific antibody in 200µl Human, Mouse, Rat $411.00 Elite

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our US partner Abcore LLC.

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Now shipping direct to US customers! 99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]), fax (888-841-9041), phone (888-320-4628). Please include the following information with your order:

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In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day.

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB13098 1
EB13098 (0.3µg/ml) staining of U2OS (A) and A549 (B) and (0.1ug/ml) HeLa (C) and KNRK (D) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB13098 2
EB13098 (0.1µg/ml) staining of Mouse Brain lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB13098 3
EB13098 (4µg/ml) staining of paraffin embedded Human Colon. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
EB13098 4
EB13098 Negative Control showing staining of paraffin embedded Human Colon, with no primary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names:
Official Symbol: RSPO3
Accession Number(s): NP_116173.2
Human GeneID(s): 84870

Immunogen

Peptide with sequence SGYYGTRYPDINKC, from the N Terminus of the protein sequence according to NP_116173.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 37kDa band observed in lysates of cell lines HeLa, U2OS, A549 and KNRK, and in Mouse Brain lysates (calculated MW of 30.9kDa according to Human NP_116173.2, and 31.4kDa according to Mouse NP_082627.3, and Rat NP_001094460.1). This molecular weight is observed by other sources, and was successfully blocked by incubation with the immunising peptide. Recommended concentration: 0.1-0.3ug//ml. Primary incubation 1 hour at room temperature.
IHC: Paraffin embedded Human Colon. Recommended concentration: 4-6µg/ml. Positive staining was also seen in paraffin embedded Human Kidney at 4ug/ml.

Species Reactivity

Tested: Human, Mouse, Rat
Expected from sequence similarity: Human, Mouse, Rat

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