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Goat Anti-Actin-like 7B (mouse) Antibody (EB07651)

Related Products: EBP07651 - Immunizing Peptide, EBP08210 - Immunizing Peptide, Goat Anti-Actin-like 7B Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB07651 Goat Anti-Actin-like 7B (mouse) Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Rat $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB07651 1
EB07651 (0.5µg/ml) staining of Rat Testes lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: actin-like 7B, ACTL7B, MGC151192, MGC151194, Tact1, actin-like 7-beta, t-actin 1
Official Symbol: Actl7b
Accession Number(s): NP_079547.1
Non-Human GeneID(s): 11471 (mouse), 313183 (rat)
Important Comments: This antibody is not expected to cross-react with Actin-like 7A.

Immunogen

Peptide with sequence C-DELHVDYELPDGK, from the internal region of the protein sequence according to NP_079547.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 45kDa band observed in Rat Testis lysates (calculated MW of 45.5kDa according to Rat NP_001020588.1 and 45.6kDa according to Mouse NP_079547.1). Recommended concentration: 0.5-1.5µg/ml. An additional band of unknown identity was also consistently observed at 30kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Species Reactivity

Tested: Rat
Expected from sequence similarity: Mouse, Rat

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