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Goat Anti-ALDH1A1 (C Terminus) Antibody - Trial (EB05049-T)
| Code | Name | Applications | Availability | Size | Species reactivity | Price | Grade | |
|---|---|---|---|---|---|---|---|---|
| EB05049-T | Goat Anti-ALDH1A1 (C Terminus) Antibody - Trial | Pep-ELISA, WB, IHC |  |
20µg specific antibody in 40µl | Human, Mouse, Rat, Dog, Cow | $105.00 |  |
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Target Protein
Principal Names: retinaldehyde dehydrogenase 1, retinal dehydrogenase 1, aldehyde dehydrogenase, liver cytosolic, aldehyde dehydrogenase 1A1, aldehyde dehydrogenase 1, soluble, acetaldehyde dehydrogenase 1, ALDH class 1, RALDH1, PUMB1, MGC2318,, ALDH11, ALDH1, ALDH-E1, ALDC, aldehyde dehydrogenase 1 family, member A1, ALDH1A1Official Symbol: ALDH1A1
Accession Number(s): NP_000680.2
Human GeneID(s): 216
Non-Human GeneID(s): 11668 (mouse), 24188 (rat)
Important Comments: This antibody may cross-react with ALDH1A2 (GeneID 8854) with one residue difference from this design and with ALDH2 (GeneID 217) with two residues difference from this design.
Immunogen
Peptide with sequence C-EVKTVTVKISQKNS, from the C Terminus of the protein sequence according to NP_000680.2.
Please note the peptide is available for sale.
Purification and Storage
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Tested
Peptide ELISA: antibody detection limit dilution 1:16000.Western blot: Approx 55kDa band observed in human liver lysates (calculated MW of 54.9kDa according to NP_000680.2). Recommended concentration: 0.03-0.1µg/ml.
IHC: This antibody has been successfully used in IHC on mouse Peyer's patches as described in the following paper: Iwata et al, Immunity. 2004 Oct;21(4):527-38.; PMID: 15485630.
Species Reactivity
Tested: Human, Mouse, RatExpected from sequence similarity: Human, Mouse, Rat, Dog, Cow
Specific References
The goat polyclonal antibody used in the following papers was manufactured by us:
Yoon M, Madden MC, Barton HA.
Developmental expression of aldehyde dehydrogenase in rat: a comparison of
liver and lung development.
Toxicol Sci. 2006 Feb;89(2):386-98.
PMID: 16291827
Dylla SJ, Beviglia L, Park IK, Chartier C, Raval J, Ngan L, Pickell K, Aguilar J, Lazetic S, Smith-Berdan S, Clarke MF, Hoey T, Lewicki J, Gurney AL.
Colorectal cancer stem cells are enriched in xenogeneic tumors following chemotherapy.
PLoS One. 2008 Jun 18;3(6):e2428.
PMID: 18560594
Szatmari I, Pap A, Rühl R, Ma JX, Illarionov PA, Besra GS, Rajnavolgyi E, Dezso B, Nagy L.
PPARgamma controls CD1d expression by turning on retinoic acid synthesis in developing human dendritic cells.
J Exp Med. 2006 Oct 2;203(10):2351-62.
PMID: 16982809
Iwata M, Hirakiyama A, Eshima Y, Kagechika H, Kato C, Song SY.
Retinoic acid imprints gut-homing specificity on T cells.
Immunity. 2004 Oct;21(4):527-38.
PMID: 15485630