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Goat Anti-ALDH3A2 Antibody (EB10132)

Related Products: EBP10132 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB10132 Goat Anti-ALDH3A2 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human, Rat $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
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Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB10132 1
EB10132 (0.03µg/ml) staining of Rat Liver lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB10132 2
EB10132 (5µg/ml) staining of paraffin embedded Human Adrenal Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: alcohol dehydrogenase family 3, subfamily A2, aldehyde dehydrogenase 3 family, member A2, aldehyde dehydrogenase 3A2, aldehyde dehydrogenase 4, aldehyde dehydrogenase family 3 member A2, aldehyde dehydrogenase family 3, subfamily A2, Aldh4, FALDH, Aldh3a2
Official Symbol: Aldh3a2
Accession Number(s): NP_113919.2
Non-Human GeneID(s): 11671 (mouse), 65183 (rat)
Important Comments: This antibody is expected to recognize both reported isoforms (NP_001026976.1; NP_000373.1).

Immunogen

Peptide with sequence C-KGLKGESVNKLRY, from the internal region of the protein sequence according to NP_113919.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 55kDa band observed in Rat Liver lysates (calculated MW of 54.1kDa according to Rat NP_113919.2). Recommended concentration: 0.03-0.1µg/ml. Primary incubation was 1 hour.
IHC: Paraffin embedded Human Adrenal Gland. Recommended concentration: 5µg/ml.

Species Reactivity

Tested: Human, Rat
Expected from sequence similarity: Mouse, Rat

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