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Code Name Applications Availability Size Tested species Price Grade  
EB13107 Goat Anti-pan ADH (N terminus) Antibody Pep-ELISA, WB Allow up to 10 days for delivery 100µg specific antibody in 200µl Human, Rat $411.00 Elite

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$73.00: Overnight shipping (not applicable to SB-shifix) to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our US partner Abcore LLC.

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In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day.

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB13107 1
EB13107 (0.3ug/ml) staining of Human Liver (A), (0.5ug/ml) Duodenum (B) and (1ug/ml) Breast (C) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB13107 2
EB13107 (0.5ug/ml) staining of Rat Liver lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: pan ADH, ADH1B, ADH2, alcohol dehydrogenase IB (class I), beta polypeptide, ADH, beta subunit, aldehyde reductase, alcohol dehydrogenase 2, alcohol dehydrogenase 2 (class I), beta polypeptide, alcohol dehydrogenase 1B (class I), beta polypeptide
Official Symbol: ADH1A, B, C
Accession Number(s): NP_000659.2; NP_000658.1; NP_000660.1
Human GeneID(s): 124 , 125
Important Comments: This is an alternative product to EB06599. This antibody is expected to recognise the alpha (ADH1A, NP_ 000658.1), the beta (ADH1B, NP_000659.2) and gamma (ADH1C, NP_000660.1) polypeptide variants of human alcohol dehydrogenase.

Immunogen

Peptide with sequence STAGKVIKCKAC, from the N Terminus of the protein sequence according to NP_000659.2; NP_000658.1; NP_000660.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:4000.
Western blot: Approx 38kDa band observed in Human Liver, Duodenum and Breast lysates and approx. 37kDa in Rat Liver lysates (calculated MW of 39.9kDa according to Human NP_000659.2 and 39.6kDa Rat NP_062159.3). Recommended concentration: 0.3-1µg/ml. Primary incubation 1 hour at room temperature.

Species Reactivity

Tested: Human, Rat
Expected from sequence similarity: Human, Mouse, Rat

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