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Goat Anti-APBB1 / FE65 Antibody (EB07373)
| Code | Name | Applications | Availability | Size | Tested species | Price | Grade | |
|---|---|---|---|---|---|---|---|---|
| EB07373 | Goat Anti-APBB1 / FE65 Antibody | Pep-ELISA, WB |  |
100µg specific antibody in 200µl | Mouse | $423.00 |  |
|
Ordering Information for United States of America (change)Order direct from Everest
$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories. |
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EB07373 (0.1µg/ml) staining of NIH/3T3 lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
Target Protein
Principal Names: APBB1, FE65, amyloid beta (A4) precursor protein-binding, family B, member 1 (Fe65), MGC:9072, RIR, adaptor protein FE65a2, amyloid beta A4 precursor protein-binding, family B, member 1, stat-like proteinOfficial Symbol: APBB1
Accession Number(s): NP_001155.1; NP_663722.1
Human GeneID(s): 322
Important Comments: This antibody is expected to recognise both reported isoforms (NP_001155.1 and NP_663722.1).
Immunogen
Peptide with sequence C-GSLKPKRLGAHTP, from the C Terminus of the protein sequence according to NP_001155.1; NP_663722.1.
Please note the peptide is available for sale.
Purification and Storage
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Tested
Peptide ELISA: antibody detection limit dilution 1:128000.Western blot: Approx 100kDa band observed in lysates of NIH/3T3 (calculated MW of 77.2kDa according to NP_001155.1 and 77.0kDa according to NP_663722.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Zambrano et al, J Biol Chem. 1998 Aug 7;273(32):20128-33.; PMID: 9685356). Recommended concentration: 0.1-0.3µg/ml. Primary incubation was 1 hour.
Species Reactivity
Tested: MouseExpected from sequence similarity: Human