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Goat Anti-14-3-3 sigma / Stratifin, Biotinylated Antibody (EB07061-B)

Related Products: EBP07061-B - Immunizing Peptide, EBP07061 - Immunizing Peptide, Goat Anti-14-3-3 sigma / Stratifin Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB07061-B Goat Anti-14-3-3 sigma / Stratifin, Biotinylated Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human, Mouse $411.00 Elite

Ordering Information for

Order direct from Everest

$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]) Please include the following information with your order:

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB07061-B 1
Biotinylated EB07061 (0.1µg/ml) staining of Mouse Skin lysate (35µg protein in RIPA buffer), exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.
Click here to download PDF datasheet.

Target Protein

Principal Names: SFN, stratifin, YWHAS, 14-3-3 sigma, epithelial cell marker protein 1
Official Symbol: SFN
Accession Number(s): NP_006133.1
Human GeneID(s): 2810
Non-Human GeneID(s): 55948 (mouse), 313017 (rat)

Immunogen

Peptide with sequence CDLHTLSEDSYKDST., from the internal region of the protein sequence according to NP_006133.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 28kDa band observed in Mouse Skin lysates (calculated MW of 27.7kDa according to Mouse NP_061224.2). See non-biotinylated parental product's datasheet for further QC data. Recommended concentration: 0.1-0.3µg/ml.

Species Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig

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