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Code Name Applications Availability Size Tested species Price Grade  
EB06443-T Goat Anti-58KGolgi protein(Internal)/FTCD Antibody - Trial Pep-ELISA, WB, IF, IHC Yes 20µg specific antibody in 40µl Human, Mouse, Rat $105.00 Elite_Plus

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TestShot
EB06443-T 1
EB06443 (0.75µg/ml) staining of Human (A) and Mouse (B) and (0.5ug/ml) of Rat © Liver lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB06443-T 2
EB06443 Immunofluorescence analysis of paraformaldehyde fixed Caco-2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB06443-T 3
EB06443 (6µg/ml) staining of paraffin embedded Human Liver. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
EB06443-T 4
EB06443 Negative Control showing staining of paraffin embedded Human Liver, with no primary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: FTCD, LCHC1, formiminotransferase cyclodeaminase, formimidoyltransferase cyclodeaminase, LC1 autoantigen
Official Symbol: FTCD
Accession Number(s): NP_006648.1; NP_996848.1; NP_001307341.1
Human GeneID(s): 10841
Important Comments: Variants (NP_006648.1; NP_996848.1) encode the same protein.

Immunogen

Peptide with sequence CLREQGRGKDQPGRL, from the internal region of the protein sequence according to NP_006648.1; NP_996848.1; NP_001307341.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 55-58kDa band observed in Human, Mouse and Rat Liver lysates (calculated MW of 58.9kDa according to Human NP_006648.1, Mouse NP_543121.1 and Rat NP_446019.1). Recommended concentration: 0.5-1µg/ml. Primary incubation 1 hour at room temperature.
IHC: Paraffin embedded Human Liver. Recommended concentration: 5-6µg/ml.

Immunofluorescence: Expression of the protein seen in the membranes and cytoplasm of Caco-2 cells. Recommended concentration: 10µg/ml. Flow Cytometry: A customer reported weak staining on Caco-2 cells at a concentration of 10ug/ml.

Species Reactivity

Tested: Human, Mouse, Rat
Expected from sequence similarity: Human, Mouse, Rat, Pig, Cow

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