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Code Name Applications Availability Size Tested species Price Grade  
EB08211 Goat Anti-ABCB5 Antibody Pep-ELISA, WB, FC Yes 100µg specific antibody in 200µl Human $315.00 Elite

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EB08211 1
EB08211 (0.3µg/ml) staining of lysates of cell line A431 (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB08211 2
Flow cytometric analysis of paraformaldehyde fixed HepG2 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: ATP-binding cassette, sub-family B (MDR/TAP), member 5, ABCB5alpha, ABCB5beta, EST422562, ATP-binding cassette, sub-family B, member 5N: P-glycoprotein ABCB5
Official Symbol: ABCB5
Accession Number(s): NP_848654.3; NP_001157413.1
Human GeneID(s): 340273
Important Comments: This antibody is expected to recognise both reported isoforms (NP_848654.3; NP_001157413.1)

Immunogen

Peptide with sequence C-QTQHRNTSKKAQ , from the internal region of the protein sequence according to NP_848654.3; NP_001157413.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 80kDa band observed in lysates of cell line A431(calculated MW of 89.8kDa according to NP_848654.3). Recommended concentration: 0.3-1µg/ml. Primary incubation was 1 hour.

Flow Cytometry: Flow cytometric analysis of HepG2 cells. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

Specific Reference

This antibody has been successfully used in Flow Cytometry in Human:
Cheung ST, Cheung PF, Cheng CK, Wong NC, Fan ST.
Granulin-epithelin precursor and ATP-dependent binding cassette (ABC)B5 regulate liver cancer cell chemoresistance.
Gastroenterology. 2011 Jan;140(1):344-55.
PMID: 20682318

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