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Goat Anti-ABCC4 / MRP4 Antibody (EB06538)

Code Name Applications Availability Size Tested species Price Grade  
EB06538 Goat Anti-ABCC4 / MRP4 Antibody Pep-ELISA, IHC Yes 100µg specific antibody in 200µl Human $315.00 Elite

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EB06538 1
EB06538 (4µg/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: ABCC4, MRP4, MOATB, MOAT-B, EST170205, ATP-binding cassette, sub-family C (CFTR/MRP), member 4, canalicular multispecific organic anion transporter (ABC superfamily), Multidrug Resistance Protein 4, RP11-74A12.1, ATP-binding cassette, sub-family C, member 4, bA464I2.1 (ATP-binding cassette, sub-family C (CFTR/MRP), member 4), canalicular multispecific organic anion transporter (ABC superfamily), multidrug resistance-associated protein 4; multispecific organic anion transporter B
Official Symbol: ABCC4
Accession Number(s): NP_005836.2
Human GeneID(s): 10257
Non-Human GeneID(s): 170924 (rat)
Important Comments: This antibody is expected to recognize isoform 1 (NP_005836.2) only.

Immunogen

Peptide with sequence CNGQPSTLTIFETAL, from the C Terminus of the protein sequence according to NP_005836.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:1000.
IHC: In paraffin embedded Human Prostate shows membranous staining in the secretory cells. Recommended concentration: 4-6µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat

Specific References

This antibody has been successfully used in IHC and WB on Mouse:
Weerachayaphorn J, Luo Y, Mennone A, Soroka CJ, Harry K, Boyer JL
Deleterious effect of oltipraz on extrahepatic cholestasis in bile duct-ligated mice.
J Hepatol. 2014 Jan;60(1):160-6
PMID: 23978715

This antibody has been successfully used in IHC on Human:
Gori I, Rodriguez Y, Pellegrini C, Achtari C, Hornung D, Chardonnens E, Wunder D, Fiche M, Canny GO.
Augmented epithelial multidrug resistance-associated protein 4 expression in peritoneal endometriosis: regulation by lipoxin A4.
Fertil Steril. 2013 Mar 6.
PMID: 23472950

This antibody has been successfully used in IHC on Human:
Montani M, Herrmanns T, Müntener M, Wild P, Sulser T, Kristiansen G
Multidrug resistance protein 4 (MRP4) expression in prostate cancer is associated with androgen signaling and decreases with tumor progression.
Virchows Arch. 2013 Apr;462(4):437-43.
PMID: 23503867

This antibody has been successfully used in WB on Mouse:
Weerachayaphorn J, Mennone A, Soroka CJ, Harry K, Hagey LR, Kensler TW, Boyer JL.
Nuclear factor-E2-related factor 2 is a major determinant of bile acid homeostasis in the liver and intestine.
Am J Physiol Gastrointest Liver Physiol. 2012 May 1;302(9):G925-36.
PMID: 22345550

This antibody has been successfully used in ICC/IF on Human:
Xu S, Weerachayaphorn J, Cai SY, Soroka CJ, Boyer JL.
Aryl hydrocarbon receptor and NF-E2-related factor 2 are key regulators of human MRP4 expression.
Am J Physiol Gastrointest Liver Physiol. 2010 Jul;299(1):G126-35.
PMID: 20395535

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