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Goat Anti-ABCE1 / RNase L Antibody (EB08737)

Code Name Applications Availability Size Tested species Price Grade  
EB08737 Goat Anti-ABCE1 / RNase L Antibody Pep-ELISA, WB, IF, FC Yes 100µg specific antibody in 200µl Human $341.00 Elite

Ordering Information for

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our US partner Abcore LLC.

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In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day.

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Order from our local distributor

ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com

EB08737 1
EB08737 (2µg/ml) staining of Human Tonsil lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB08737 2
EB08737 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB08737 3
EB08737 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB08737 4
EB08737 Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: ABCE1, ATP-binding cassette, sub-family E (OABP), member 1, ABC38, OABP, RLI, RNASEL1, RNASELI, RNS4I, ATP-binding cassette, sub-family E, member 1, RNase L inhibitor, ribonuclease L (2\1,5\1-oligoisoadenylate synthetase-dependent) inhibitor
Official Symbol: ABCE1
Accession Number(s): NP_002931.2; NP_001035809.1
Human GeneID(s): 6059
Non-Human GeneID(s): 24015 (mouse), 361390 (rat)

Immunogen

Peptide with sequence NHDKCKPKKCRQEC, from the N Terminus of the protein sequence according to NP_002931.2; NP_001035809.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 70kDa band observed in Human Tonsil lysates and in preliminary testing of Human Ovary lysate (calculated MW of 67.3kDa according to Human NP_002931.2). Recommended concentration: 1-2ug/ml. Primary incubation 1 hour at room temperature.

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of A431 and U2OS cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

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