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Code Name Applications Availability Size Tested species Price Grade  
EB10204 Goat Anti-ACHRE / CHRNE Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Mouse $411.00 Elite

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB10204 1
EB10204 (1µg/ml) staining of Mouse Fetal Skeletal Muscle lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: AchR epsilon subunit, ACHRE, cholinergic receptor, nicotinic, epsilon, cholinergic receptor, nicotinic, epsilon polypeptide, CMS1D, CMS1E, CMS2A, FCCMS, nicotinic acetylcholine receptor epsilon polypeptide, SCCMS, CHRNE
Official Symbol: CHRNE
Accession Number(s): NP_000071.1
Human GeneID(s): 1145
Non-Human GeneID(s): 11448 (mouse), 29422 (rat)

Immunogen

Peptide with sequence C-DQEATGEEVSD, from the Internal region of the protein sequence according to NP_000071.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:1000.
Western blot: Approx 55kDa band observed in Mouse Fetal Skeletal Muscle lysates (calculated MW of 54.9kDa according to NP_033733.1). Recommended concentration: 1-3µg/ml.

Species Reactivity

Tested: Mouse
Expected from sequence similarity: Human, Mouse, Rat, Cow

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