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Code Name Applications Availability Size Tested species Price Grade  
EB11060 Goat Anti-Adam12 (mouse) Antibody Pep-ELISA Yes 100µg specific antibody in 200µl $248.00 Aspiring

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Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
Click here to download PDF datasheet.

Target Protein

Principal Names: a disintegrin and metallopeptidase domain 12 (meltrin alpha), a disintegrin and metalloprotease domain 12, a disintegrin and metalloproteinase domain 12 (meltrin alpha), ADAM 12, disintegrin and metalloproteinase domain-containing protein 12, KIAA4001, meltrin alpha, meltrin-alpha, mKIAA4001, Mltna, OTTMUSP00000023508, Adam12
Official Symbol: Adam12
Accession Number(s): NP_031426.2
Human GeneID(s): 8038
Non-Human GeneID(s): 11489 (mouse), 679837 (rat)
Important Comments: This antibody is expected to recognize both reported isoforms (NP_003465.3; NP_067673.2).

Immunogen

Peptide with sequence KKTTMEKLRCVHP, from the internal region of the protein sequence according to NP_031426.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:4000.
Western blot: Preliminary experiments gave an approx 37kDa band in Mouse and Rat Skeletal Muscle lysates after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 98.5kDa according to NP_031426.2. The 37kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Species Reactivity

Tested:
Expected from sequence similarity: Mouse, Rat, Pig

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