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Goat Anti-ADRB2R / ADRB2 Antibody (EB06937)

Code Name Applications Availability Size Tested species Price Grade  
EB06937 Goat Anti-ADRB2R / ADRB2 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $341.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com

EB06937 1
EB06937 (0.5µg/ml) staining of HepG2 cell ysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: ADRB2, ADRB2R, adrenergic, beta-2-, receptor, surface [Homo sapiens], HGNC:286, ADRBR, B2AR, BAR, BETA2AR, beta-2 adrenergic receptor, beta-2 adrenoceptor, catecholamine receptor
Official Symbol: ADRB2
Accession Number(s): NP_000015.1
Human GeneID(s): 154

Immunogen

Peptide with sequence C-HQGTVPSDNIDSQ, from the C Terminus of the protein sequence according to NP_000015.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 48kDa band observed in lysates of cell lind HepG2 (calculated MW of 46.6kDa according to NP_000015.1). Recommended concentration: 0.3-1µg/ml. Primary incubation 1 hour at room temperature.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Dog, Cow

Specific Reference

This antibody (previous batch) has been successfully used in Western blot on Cow:
Rekawiecki R, Nowocin A, Kotwica J.
Relationship between concentrations of progesterone, oxytocin, noradrenaline, gene expression and protein level for their receptors in corpus luteum during estrous cycle in the cow.
Prostaglandins Other Lipid Mediat. 2010 Jun;92(1-4):13-8.
PMID: 20149890

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