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Goat Anti-AGXT / AGT Antibody (EB09063)

Code Name Applications Availability Size Tested species Price Grade  
EB09063 Goat Anti-AGXT / AGT Antibody Pep-ELISA, IF, FC Yes 100µg specific antibody in 200µl Human $341.00 Elite

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Now shipping direct to US customers! 99% of products are held in stock for immediate shipment, see individual product pages for availability.

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In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day.

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com

EB09063 1
EB09063 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing vesicle staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB09063 2
EB09063 Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing vesicle staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB09063 3
EB09063 Flow cytometric analysis of paraformaldehyde fixed HepG2 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: AGXT, alanine-glyoxylate aminotransferase, AGT, AGT1, AGXT1, PH1, SPAT, SPT, TLH6, L-alanine: glyoxylate aminotransferase 1, hepatic peroxisomal alanine:glyoxylate aminotransferase, serine-pyruvate aminotransferase, serine:pyruvate aminotransferase
Official Symbol: AGXT
Accession Number(s): NP_000021.1
Human GeneID(s): 189

Immunogen

Peptide with sequence C-DKAKKKMYSRK, from the internal region of the protein sequence according to NP_000021.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:2000.

Immunofluorescence: Strong expression of the protein seen in the vesicles of HeLa and HepG2 cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of HepG2 cells. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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