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Goat Anti-AKR1C4 Antibody (EB07240)

Related Products: EBP07240 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB07240 Goat Anti-AKR1C4 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB07240 1
EB07240 (0.1µg/ml) staining of human liver lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB07240 2
EB07240 (2.5µg/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: AKR1C4, aldo-keto reductase family 1, member C4, HGNC:387, 3-alpha-HSD, C11, CDR, CHDR, DD4, HAKRA, MGC22581, 3-alpha hydroxysteroid dehydrogenase, type I, 3 alpha-hydroxysteroid dehydrogenase/dihydrodiol dehydrogenase 4, 3-alpha-hydroxysteroid dehydrogenase variant, aldo-keto reductase family 1, member C4, chlordecone reductase, dihydrodiol dehydrogenase 4, dihydrodiol dehydrogenase isozyme DD4, type I 3-alpha-hydroxysteroid dehydrogenase
Official Symbol: AKR1C4
Accession Number(s): NP_001809.2
Human GeneID(s): 1109
Important Comments: This antibody may cross-react with AKR1C1

Immunogen

Peptide with sequence DPKYQRVELNDGH-C, from the N Terminus of the protein sequence according to NP_001809.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:4000.
Western blot: Approx. 35kDa band observed in Human Liver lysates (calculated MW of 37.1kDa according to NP_001809.2). Recommended concentration: 0.1-0.3µg/ml.
IHC: In paraffin embedded Human Liver shows staining of distinct structures in the cytoplasm of hepatocytes. Recommended concentration, 2-4µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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