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Code Name Applications Availability Size Tested species Price Grade  
EB05049 Goat Anti-ALDH1A1 (C Terminus) Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human, Mouse, Rat $324.00 Elite

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EB05049 1
EB05049 (0.1µg/ml) staining of Human (A), Mouse (B) and Rat (C) Liver lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: ALDH1A1, aldehyde dehydrogenase 1 family, member A1, ALDC, ALDH-E1, ALDH1, ALDH11, MGC2318,, PUMB1, RALDH1, ALDH class 1, acetaldehyde dehydrogenase 1, aldehyde dehydrogenase 1, soluble, aldehyde dehydrogenase 1A1, aldehyde dehydrogenase, liver cytosolic, retinal dehydrogenase 1, retinaldehyde dehydrogenase 1
Official Symbol: ALDH1A1
Accession Number(s): NP_000680.2
Human GeneID(s): 216
Non-Human GeneID(s): 11668 (mouse), 24188 (rat)
Important Comments: This antibody may cross-react with ALDH1A2 (GeneID 8854) with one residue difference from this design and with ALDH2 (GeneID 217) with two residues difference from this design.

Immunogen

Peptide with sequence C-EVKTVTVKISQKNS, from the C Terminus of the protein sequence according to NP_000680.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:1000.
Western blot: Approx 55kDa band observed in Human, Mouse and Rat Liver lysates (calculated MW of 54.9kDa according to NP_000680.2). Recommended concentration: 0.1-0.3µg/ml. Primary incubation was 1 hour.

Species Reactivity

Tested: Human, Mouse, Rat
Expected from sequence similarity: Human, Mouse, Rat

Specific Reference

This antibody (previous batch) has been successfully used in WB and ICC on Human:
Amann PM, Hofmann C, Freudenberger M, Holland-Cunz S, Eichmüller SB, Bazhin AV.
Expression and activity of alcohol and aldehyde dehydrogenases in melanoma cells and in melanocytes.
J Cell Biochem. 2012 Mar;113(3):792-9.
PMID: 22020955

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