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Goat Anti-Alfy / WDFY3 Antibody (EB12545)

Related Products: EBP12545 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB12545 Goat Anti-Alfy / WDFY3 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Mouse $411.00 Elite

Ordering Information for

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our US partner Abcore LLC.

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Now shipping direct to US customers! 99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]), fax (888-841-9041), phone (888-320-4628). Please include the following information with your order:

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In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day.

Orders are shipped by our US logistics partner Abcore LLC.

 

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Order from our local distributor

ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB12545 1
EB12545 (5µg/ml) staining of Mouse Brain lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: WDFY3, WD repeat and FYVE domain containing 3, ALFY, ZFYVE25, WD repeat and FYVE domain-containing protein 3, autophagy-linked FYVE protein
Official Symbol: WDFY3
Accession Number(s): NP_055806.2
Human GeneID(s): 23001
Non-Human GeneID(s): 72145 (mouse), 305164 (rat)

Immunogen

Peptide with sequence C-SPERSTRTQQKEFQT, from the internal region of the protein sequence according to NP_055806.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 400kDa band observed in Mouse Brain lysates (calculated MW of 395kDa according to NP_055806.2). Data obtained from anonymous customer. The lower molecular weight bands are non-specific. We call for caution when used for other assays than Western blot. Recommended concentration: 4-6µg/ml.

Species Reactivity

Tested: Mouse
Expected from sequence similarity: Human, Mouse, Rat, Cow

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