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Goat Anti-ALS Antibody (EB09337)
| Code | Name | Applications | Availability | Size | Tested species | Price | Grade | |
|---|---|---|---|---|---|---|---|---|
| EB09337 | Goat Anti-ALS Antibody | Pep-ELISA |  |
100µg specific antibody in 200µl | $255.00 |  |
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Ordering Information for United States of America (change)Order direct from Everest
$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories. |
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Target Protein
Principal Names: IGFALS, insulin-like growth factor binding protein, acid labile subunit, ALS, INSULIN-LIKE GROWTH FACTOR BINDING PROTEIN COMPLEX ACID LABILE CHAINOfficial Symbol: IGFALS
Accession Number(s): NP_004961.1
Human GeneID(s): 3483
Non-Human GeneID(s): 16005 (mouse), 79438 (rat)
Immunogen
Peptide with sequence C-GLDLRDLSEAH, from the C Terminus of the protein sequence according to NP_004961.1.
Please note the peptide is available for sale.
Purification and Storage
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Tested
Peptide ELISA: antibody detection limit dilution 1:16000.Western blot: Preliminary experiments gave an approx 45kDa band in lysates of cell line HepG2 after 2µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 66.0kDa according to NP_004961.1. The 45kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Species Reactivity
Tested:Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow, Pig