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Goat Anti-ALS2CR3 / GRIF1 Antibody (EB05870)

Related Products: EBP05870 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB05870 Goat Anti-ALS2CR3 / GRIF1 Antibody Pep-ELISA, FC, IF Yes 100µg specific antibody in 200µl Human $423.00 Elite

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EB05870 1
EB05870 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
EB05870 2
EB05870 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml). Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
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Target Protein

Principal Names: TRAK2, ALS2CR3, amyotrophic lateral sclerosis 2 (juvenile) chromosome region, candidate 3, GRIF1, CALS-C, GRIF-1, KIAA0549, likely ortholog of rat GABA(A) receptor-associated protein (GRIF-1), trafficking protein, kinesin binding 2, OIP98
Official Symbol: TRAK2
Accession Number(s): NP_055864.2
Human GeneID(s): 66008

Immunogen

Peptide with sequence CTSSPKMGVLKED, from the C Terminus of the protein sequence according to NP_055864.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.

Immunofluorescence: Strong expression of the protein seen in U2OS cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Pig, Cow