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Goat Anti-Amisyn / STXBP6 Antibody (EB05935)

Related Products: EBP05935 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB05935 Goat Anti-Amisyn / STXBP6 Antibody Pep-ELISA, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
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EB05935 1
EB05935 (3.8µg/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: STXBP6, syntaxin binding protein 6 (amisyn), amisyn, HSPC156, amisyn, syntaxin binding protein 6, FLJ39638, OTTHUMP00000178754
Official Symbol: STXBP6
Accession Number(s): NP_054897.4
Human GeneID(s): 29091
Non-Human GeneID(s): 217517 (mouse)

Immunogen

Peptide with sequence SAKSAISKEIFAP-C, from the N Terminus of the protein sequence according to NP_054897.4.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Preliminary experiments gave bands at both approx 25kDa and approx 45kDa in Human Brain extracts at 0.1µg/ml. Please note that currently we cannot find an explanation in the literature for the 45kDa band we observe given the predicted size of approx. 24Kda according to NP_054897. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any splice variants/modified forms been reported?
IHC: In paraffin embedded Human Kidney shows cytoplasm staining of PCT and DCT cells Recommended concentration, 3-5µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

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