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Goat Anti-Annexin I Antibody (EB09185)

Related Products: EBP09185 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB09185 Goat Anti-Annexin I Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human, Mouse, Rat, Pig $411.00 Elite

Ordering Information for

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our US partner Abcore LLC.

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Now shipping direct to US customers! 99% of products are held in stock for immediate shipment, see individual product pages for availability.

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In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day.

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Order from our local distributor

ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB09185 1
EB09185 (1µg/ml) staining of K562 (A) and NIH3T3 (B) lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence
EB09185 2
EB09185 (1µg/ml) staining of Rat Spleen lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB09185 3
EB09185 (1µg/ml) staining of Pig Spleen lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB09185 4
EB09185 (4µg/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. This data is from a previous batch, not on sale.
EB09185 5
EB09185 (4µg/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. This data is from a previous batch, not on sale.
Click here to download PDF datasheet.

Target Protein

Principal Names: annexin I, ANXA1, annexin A1, ANX1, LPC1, annexin I (lipocortin I), lipocortin I
Official Symbol: ANXA1
Accession Number(s): NP_000691.1
Human GeneID(s): 301
Non-Human GeneID(s): 16952 (mouse), 25380 (rat)

Immunogen

Peptide with sequence CQAILDETKGDYEK, from the C Terminus of the protein sequence according to NP_000691.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:4000.
Western blot: Approx 37kDa band observed in lysates of cell lines K562 and NIH3T3 (calculated MW of 38.7kDa according to Human NP_000691.1 and Mouse NP_034860.2). Approx 37kDa band observed in Rat and Pig Spleen lysates (calculated MW of 38.7kDa according to Pig NP_001157470.1). Recommended concentration: 1-3µg/ml.

Species Reactivity

Tested: Human, Mouse, Rat, Pig
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow, Pig

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