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Goat Anti-AOC3 / VAP1 Antibody (EB09865)

Code Name Applications Availability Size Tested species Price Grade  
EB09865 Goat Anti-AOC3 / VAP1 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $411.00 Elite

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 

EB09865 1
EB09865 (0.1µg/ml) staining of A549 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: amine oxidase, copper containing 3, amine oxidase, copper containing 3 (vascular adhesion protein 1), copper amine oxidase, HPAO, semicarbazide-sensitive amine oxidase, SSAO, VAP1, VAP-1, vascular adhesion protein 1, AOC3
Official Symbol: AOC3
Accession Number(s): NP_003725.1
Human GeneID(s): 8639

Immunogen

Peptide with sequence ESQAPKTIRDAFC, from the internal region of the protein sequence according to NP_003725.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 90kDa band observed in lysates of cell line A549 (calculated MW of 84.6kDa according to NP_003725.1). Recommended concentration: 0.1-0.3µg/ml. An additional band of unknown identity was also consistently observed at 38kDa. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat

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