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Code Name Applications Availability Size Tested species Price Grade  
EB11875 Goat Anti-AP2A1 (aa706-727) Antibody Pep-ELISA, WB, IHC, ICC Yes 100µg specific antibody in 200µl Human, Mouse $315.00 Elite

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EB11875 1
EB11875 (0.01µg/ml) staining of Human Frontal Cortex lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB11875 2
EB11875 (5µg/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB11875 3
EB11875 (0.1µg/ml) staining of Mouse Brain lysate (~5µg protein in SDSPAGE buffer). The left panel shows the same blot stained with Ponceau red for total protein stain as the loading control before labelling. Primary incubation was 1 hour. Detected by chemiluminescence. Data obtained from Prof. M Robinson, CIMR, Cambridge, UK.
EB11875 4
EB11875 (0.1µg/ml) staining of methanol-fixed HeLa cells with stably expressing Mouse Ap2a1. Primary incubation was 1 hour. Detected by Alexa Fluor 594. Data obtained from Prof. M Robinson, CIMR, Cambridge, UK.
Click here to download PDF datasheet.

Target Protein

Principal Names: AP2A1, adaptor-related protein complex 2, alpha 1 subunit, ADTAA, AP2-ALPHA, CLAPA1, 100 kDa coated vesicle protein A, AP-2 complex subunit alpha-1, adapter-related protein complex 2 alpha-1 subunit, adaptin, alpha A, adaptor protein complex AP-2 subunit alpha-1, alpha-adaptin A, alpha1-adaptin, clathrin assembly protein complex 2 alpha-A large chain, clathrin-associated/assembly/adaptor protein, large, alpha 1, plasma membrane adaptor HA2/AP2 adaptin alpha A subunit
Official Symbol: AP2A1
Accession Number(s): NP_055018.2
Human GeneID(s): 160
Non-Human GeneID(s): 11771 (mouse), 308578 (rat)
Important Comments: This antibody is expected to recognize reported isoform 1 (NP_055018.2) only.

Immunogen

Peptide with sequence C-ELEPPAPESPMALLADPAPAAD, from the internal region of the protein sequence according to NP_055018.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 110kDa band observed in Human Brain (Frontal Cortex) lysates (calculated MW of 108kDa according to NP_055018.2). The same band is observed in Mouse Brain, but not in Mouse Liver or Mouse Kidney lysates. Recommended concentration: 0.01-0.03µg/ml.
IHC: Paraffin embedded Human Brain (Cortex). Recommended concentration: 5µg/ml.

Immunocytochemistry: HeLa stably expressing a chimeric construct including the brain-specific splice variant of Mouse Ap2a1, showing clathrin-coated pits and vesicles budding from the plasma membrane.

Species Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Rat, Pig, Cow

Specific Reference

This antibody has been successfully used in Western blot on Human:
Dominik Pascal Buser, Marie-Françoise Ritz, Suzette Moes; Cristobal Tostado, Stephan Frank, Martin Spiess, Luigi Mariani, Paul Jenö, Jean-Louis Boulay, Gregor Hutter
Quantitative proteomics reveals reduction of endocytic machinery components in gliomas
https://doi.org/10.1016/j.ebiom.2019.07.039
PMID: 0

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