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Code Name Applications Availability Size Tested species Price Grade  
EB07590 Goat Anti-APPL1 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl $411.00 Elite

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$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB07590 1
EB07590 (0.5µg/ml) staining of 1) Rat Aortic Smooth Muscle cell lysate 2) after infection with Human APPL1 adenovirus for 48hrs. Detected by chemiluminescence. Data kindly provided by Dr. L. Jia, Ann Arbor, USA.
Click here to download PDF datasheet.

Target Protein

Principal Names: APPL1, DIP13alpha, adaptor protein containing pH domain, PTB domain and leucine zipper motif, signaling adaptor protein DIP13alpha
Official Symbol: APPL1
Accession Number(s): NP_036228.1
Human GeneID(s): 26060
Non-Human GeneID(s): 72993 (mouse)

Immunogen

Peptide with sequence C-NRYSRLSKKRENDK, from the internal region of the protein sequence according to NP_036228.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx 80kDa band observed in Rat Aortic Smooth Skeletal Muscle Cells (lane 1), intensity increased after infection with Human APPL1 adenovirus for 48h (lane 2). Calculated MW of 79.7kDa according to NP_036228.1. The blot was co-labeled with Tubulin-specific antibodies as a loading control. Recommended concentration: 0.3-1µg/ml.

Species Reactivity

Tested:
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

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