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|EB06745-T||Goat Anti-Arrestin beta 2 Antibody - Trial||Pep-ELISA, WB, IF, FC||20µg specific antibody in 40µl||Human||$105.00|
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Peptide with sequence C-HDHIPLPRPQS, from the internal region of the protein sequence according to NP_004304.1; NP_945355.1; NP_001244257.1; NP_001244259.1; NP_001244260.1; NP_001316993.1.
Please note the peptide is available for sale.
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.
Immunofluorescence: Strong expression of the protein seen in HeLa cells. Recommended concentration: 10µg/ml.
Flow Cytometry: Flow cytometric analysis of A549 cells. Recommended concentration: 10ug/ml.
This antibody (previous batch) has been successfully used in IP on Rat:
Zakrzewicz A, Krasteva G, Wilhelm J, Dietrich H, Wilker S, Padberg W, Wygrecka M, Grau V.
Reduced expression of arrestin beta 2 by graft monocytes during acute rejection of rat kidneys.
Immunobiology. 2011 Jul;216(7):854-61.
This antibody (previous batch) has been successfully used in WB on Mouse:
Napolitano F, Pasqualetti M, Usiello A, Santini E, Pacini G, Sciamanna G, Errico F, Tassone A, Di Dato V, Martella G, Cuomo D, Fisone G, Bernardi G, Mandolesi G, Mercuri NB, Standaert DG, Pisani A.
Dopamine D2 receptor dysfunction is rescued by adenosine A2A receptor antagonism in a model of DYT1 dystonia.
Neurobiol Dis. 2010 Jun;38(3):434-45.