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Goat Anti-ATF2 Antibody - Trial (EB05198-T)

Related Products: EBP05198 - Immunizing Peptide, Goat Anti-ATF2 Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB05198-T Goat Anti-ATF2 Antibody - Trial Pep-ELISA, WB Yes 20µg specific antibody in 40µl Human $105.00 Elite

Ordering Information for

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$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]) Please include the following information with your order:

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
TestShot
EB05198-T 1
EB05198 (1µg/ml) staining of HepG2 (A), K562 (B) and HeLa (C) nuclear lysates (35µg protein in RIPA buffer). Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: ATF2, activating transcription factor 2, HB16, CREB2, TREB7, CRE-BP1, cAMP responsive element binding protein 2, MGC111558, activating transcription factor 2 splice variant ATF2-var2, cAMP responsive element binding protein 2, formerly
Official Symbol: ATF2
Accession Number(s): NP_001871.2
Human GeneID(s): 1386
Non-Human GeneID(s): 81647 (rat)

Immunogen

Peptide with sequence C-IVMAPSSQSQPSGS, from the C Terminus of the protein sequence according to NP_001871.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 75kDa observed in nuclear lysates of cell lines HeLa, HepG2 and K562. This molecular weight is routinely observed by other sources. Recommended concentration: 0.3-1µg/ml. Primary incubation 1 hour at room temperature.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Rat, Dog, Cow

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