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Goat Anti-ATF7 Antibody (EB05244)

Code Name Applications Availability Size Tested species Price Grade  
EB05244 Goat Anti-ATF7 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $324.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: info@arp1.com
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EB05244 1
EB05244 (3.8µg/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB05244 2
EB05244 staining (0.5µg/ml) of HeLa lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: ATF7, ATFA, activating transcription factor 7, MGC57182
Official Symbol: ATF7
Accession Number(s): NP_001123531.1; NP_006847.1; NP_001123532.1
Human GeneID(s): 11016
Important Comments: This antibody is expected to recognise all three reported isoforms (NP_001123531.1, NP_006847.1 and NP_001123532.1).

Immunogen

Peptide with sequence C-HVIMTPQSQSAGR, from the C Terminus of the protein sequence according to NP_001123531.1; NP_006847.1; NP_001123532.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 35kDa band seen in HeLa cell lysates. Recommended for use at 0.5-2µg/ml. Please note the band size is in agreement with previous experimental data (J Biol Chem 2001 Apr 27;276(17):13718-26).
IHC: In paraffin embedded Human Testis shows staining of select spermatocytes in Human Testis. Recommended concentration, 3-5µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Rat, Dog, Cow

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