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Goat Anti-ATG16L1 Antibody (EB07893)

Related Products: EBP07893 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB07893 Goat Anti-ATG16L1 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Mouse, Human $411.00 Elite

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]) Please include the following information with your order:

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB07893 1
EB07893 (0.1µg/ml) staining of Mouse Brain lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB07893 2
EB07893 (3.75µg/ml) staining of paraffin embedded Human Skin. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: ATG16, autophagy related 16-like 1 (S. cerevisiae), hCG_1817841, APG16L, ATG16L, FLJ00045, FLJ10035, FLJ10828, FLJ22677, WDR30, APG16 autophagy 16-like, APG16L beta, ATG16 autophagy related 16-like protein 1, WD repeat domain 30
Official Symbol: ATG16L1
Accession Number(s): NP_110430.5; NP_060444.3
Human GeneID(s): 55054
Important Comments: This antibody is expected to recognise both reported isoforms (NP_110430.5 and NP_060444.3).

Immunogen

Peptide with sequence C-KVEKVLSKQHSSSIN, from the internal region (near the C Terminus) of the protein sequence according to NP_110430.5; NP_060444.3.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 65kDa band observed in Mouse Brain lysates (calculated MW of 68.1kDa according to Mouse NP_084122.2). Recommended concentration: 0.01-0.1µg/ml. Primary incubation was 1 hour.
IHC: Paraffin embedded Human Skin. Recommended concentration: 3.75µg/ml.

Species Reactivity

Tested: Mouse, Human
Expected from sequence similarity: Human, Mouse, Rat, Dog

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