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Goat Anti-ATG4D Antibody (EB09223)

Code Name Applications Availability Size Tested species Price Grade  
EB09223 Goat Anti-ATG4D Antibody Pep-ELISA, IHC Yes 100µg specific antibody in 200µl Human $333.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: info@arp1.com
www.arp1.com

EB09223 1
EB09223 (2.5µg/ml) staining of paraffin embedded Human Pancreas. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: ATG4D, ATG4 autophagy related 4 homolog D (S. cerevisiae), APG4-D, APG4D, AUTL4, APG4 autophagy 4 homolog D, AUT-like 4, cysteine endopeptidase, autophagin-4, autophagy-related cysteine endopeptidase 4, cysteine protease involved in autophagy
Official Symbol: ATG4D
Accession Number(s): NP_116274.3
Human GeneID(s): 84971
Non-Human GeneID(s): 235040 (mouse), 686505 (rat)

Immunogen

Peptide with sequence CSQLAQPTLRLPRT, from the internal region (near C terminus) of the protein sequence according to NP_116274.3.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Preliminary experiments gave an approx. 26kDa band in Human Skeletal Muscle lysates after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 52.9kDa according to NP_116274.3. The 26kDa band was successfully blocked by incubation with the immunizing peptide.
IHC: Paraffin embedded Human Pancreas. Recommended concentration: 2.5µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig

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