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Goat Anti-ATP13A1 Antibody (EB08261)

Related Products: EBP08261 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB08261 Goat Anti-ATP13A1 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB08261 1
EB08261 (0.3µg/ml) staining of Human Kidney lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB08261 2
EB08261 (5µg/ml) staining of paraffin embedded Human Thyroid. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: ATP13A1, ATPase type 13A1, ATP13A, CGI-152, DKFZp761L1623, FLJ31858, FLJ41786, FLJ43873, FLJ90317, KIAA1825, cation transporting ATPase, cation-transporting ATPase
Official Symbol: ATP13A1
Accession Number(s): NP_065143.2
Human GeneID(s): 57130
Non-Human GeneID(s): 170759 (mouse), 290673 (rat)

Immunogen

Peptide with sequence C-REAQARSPEKQEQ, from the internal region of the protein sequence according to NP_065143.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx 110kDa band observed in Human Kidney lysates (calculated MW of 133kDa according to NP_065143.2). Recommended concentration: 0.3-1µg/ml.
IHC: Paraffin embedded Human Thyroid. Recommended concentration: 5µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog

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