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Goat Anti-Atrial natriuretic factor, Biotinylated Antibody (EB11166-B)

Code Name Applications Availability Size Tested species Price Grade  
EB11166-B Goat Anti-Atrial natriuretic factor, Biotinylated Antibody Pep-ELISA, WB, IHC Contact sales for delivery estimate 100µg specific antibody in 200µl Human, Mouse, Rat $324.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: info@arp1.com
www.arp1.com

EB11166-B 1
Biotinylated EB11166 (2µg/ml) staining of Mouse Heart lysate (35µg protein in RIPA buffer), exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.
Click here to download PDF datasheet.

Target Protein

Principal Names: NPPA, natriuretic peptide A, ANF, ANP, ATFB6, ATRST2, CDD, CDD-ANF, CDP, PND, atriopeptin, cardiodilatin-related peptide, cardionatrin, natriuretic peptide precursor A variant 1, prepronatriodilatin
Official Symbol: NPPA
Accession Number(s): NP_006163.1
Human GeneID(s): 4878
Non-Human GeneID(s): 230899 (mouse), 24602 (rat)

Immunogen

Peptide with sequence RIGAQSGLGCNSFR., from the C Terminus of the protein sequence according to NP_006163.1.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 19kDa band observed in Mouse Heart lysates (calculated MW of 16.4kDa according to NP_006163.1). See non-biotinylated parental product's datasheet for further QC data. Recommended concentration: 1-3µg/ml.

Species Reactivity

Tested: Human, Mouse, Rat
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

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