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Goat Anti-BAF57 / SMARCE1 Antibody (EB06054)

Related Products: EBP06054 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB06054 Goat Anti-BAF57 / SMARCE1 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
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www.arp1.com
EB06054 1
In paraffin embedded Human Prostate shows nuclear staining in secretory epithelial cells. Recommended concentration, 3-5µg/ml.
EB06054 2
A) HEK293 overexpressing BAF57 (RC209444) and probed with EB06054 (mock transfection in first lane), tested by Origene. B) see Western Blot.
Click here to download PDF datasheet.

Target Protein

Principal Names: SMARCE1, BAF57, SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily e, member 1, mammalian chromatin remodeling complex BRG1-associated factor 57, BAF57, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin e1
Official Symbol: SMARCE1
Accession Number(s): NP_003070.3
Human GeneID(s): 6605
Non-Human GeneID(s): 57376 (mouse)

Immunogen

Peptide with sequence C-PPTDPIPEDEKKE, from the C Terminus of the protein sequence according to NP_003070.3.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 50kDa band observed in lysates of cell lines HEPG2, HeLa and Jurkat (calculated MW of 46.6kDa according to NP_003070.3). A 48kDa band was observed in transfected HEK293 transiently expressing BAF57. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.2-0.6 µg/ml. Full caption: A) HEK293 overexpressing BAF57 (RC209444) and probed with EB06054 (mock transfection in first lane), tested by Origene. B) EB06054 staining (0.2µg/ml) of Jurkat lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
IHC: In paraffin embedded Human Prostate shows nuclear staining in secretory epithelial cells. Recommended concentration, 3-5µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse

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