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Code Name Applications Availability Size Tested species Price Grade  
EB05175 Goat Anti-BAIAP2 (isoform 3) Antibody Pep-ELISA, WB-Trf, IHC Yes 100µg specific antibody in 200µl Human $324.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: info@arp1.com
www.arp1.com

EB05175 1
HEK293 lysate (10ug protein in RIPA buffer) over expressing Human BAIAP2 with DYKDDDDK tag probed with EB05175 (1.0 ug/ml) in Lane A and probed with anti- DYKDDDDK Tag (1/3000) in lane C. Mock-transfected HEK293 probed with EB05175 (1mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.
EB05175 2
EB05175 (3.75µg/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: BAIAP2, BAP2, IRSP53, BAI1-associated protein 2, FLAF3, brain-specific angiogenesis inhibitor 1-associated protein 2, fas ligand-associated factor 3, insulin receptor substrate p53, insulin receptor substrate p53/p58
Official Symbol: BAIAP2
Accession Number(s): NP_006331.1
Human GeneID(s): 10458
Important Comments: This antibody will recognise only one of three reported isoforms (NP_006331.1).

Immunogen

Peptide with sequence C-SMSSADVEVARF, from the C Terminus of the protein sequence according to NP_006331.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:8000.
Western blot: In transfected HEK293 transiently expressing full-length Human BAIAP2 (myc and DYKDDDDK tagged), a band of approx. 60kDa was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-DYKDDDDK tag antibody. Recommended concentration, 1-2µg/ml.
IHC: Paraffin embedded Human Brain (Cortex). Recommended concentration: 3.75µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Dog, Cow

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