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Goat Anti-BEND5 Antibody (EB12141)

Code Name Applications Availability Size Tested species Price Grade  
EB12141 Goat Anti-BEND5 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human , Mouse $423.00 Elite

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EB12141 1
EB12141 (0.3µg/ml) staining of Daudi (A), Human Tonsil (B), Human Spleen (C) and Mouse Spleen (D) lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: BEND5, BEN domain containing 5, C1orf165
Official Symbol: BEND5
Accession Number(s): NP_078879.2
Human GeneID(s): 79656
Non-Human GeneID(s): 67621 (mouse), 362564 (rat)

Immunogen

Peptide with sequence CKNEERREAKYNLQ, from the C Terminus of the protein sequence according to NP_078879.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 48kDa band observed in Human Tonsil and Spleen and Mouse Spleen lysates and also in lysates of cell line Daudi (calculated MW of 48.2kDa according to NP_078879.2). Recommended concentration: 0.3-1µg/ml.

Species Reactivity

Tested: Human , Mouse
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow