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Goat Anti-BERP / RNF22 Antibody (EB05868)

Related Products: EBP05868 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB05868 Goat Anti-BERP / RNF22 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human, Mouse, Rat $411.00 Elite

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB05868 1
EB05868 (0.3µg/ml) staining of Human Cerebellum (A) and Rat (B) Brain lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence
EB05868 2
EB05868 staining (0.05µg/ml) of Mouse Brain lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: TRIM3, BERP, RNF22, TRIM3, tripartite motif-containing 3, HAC1, RNF97, ring finger protein 22, brain expressed ring finger, tripartite motif protein TRIM3, FLJ16135
Official Symbol: TRIM3
Accession Number(s): NP_006449.2; NP_150594.2
Human GeneID(s): 10612
Non-Human GeneID(s): 55992 (mouse), 83616 (rat)
Important Comments: Both variants (NP_006449.2; NP_150594.2;) represent the same protein. This antibody is expected to recognise all three human isoforms of this protein.

Immunogen

Peptide with sequence AKREDSPGPEVQP-C, from the N Terminus of the protein sequence according to NP_006449.2; NP_150594.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx 80kDa band observed in Human Brain (Cerebellum) and Rat Brain lysates and approx 85kDa band seen in Mouse Brain (calculated MW of 80.8kDa according to Human NP_006449.2, Mouse NP_001272799.1 and Rat NP_113974.1). Recommended concentration: 0.1-0.3µg/ml.

Species Reactivity

Tested: Human, Mouse, Rat
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

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