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Goat Anti-BID Antibody - Trial (EB06713-T)

Related Products: EBP06713 - Immunizing Peptide, Goat Anti-BID Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB06713-T Goat Anti-BID Antibody - Trial Pep-ELISA, WB, IF Yes 20µg specific antibody in 40µl Human $105.00 Elite

Ordering Information for

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$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]) Please include the following information with your order:

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
TestShot
EB06713-T 1
EB06713 Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB06713-T 2
EB06713 (0.1µg/ml) staining of A431 lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: BID, BH3 interacting domain death agonist, HGNC:1050, MGC15319, MGC42355, BH3-interacting domain death agonist, Human BID coding sequence, apoptic death agonist, FP497, BID isoform ES(1b), BID isoform L(2), BID isoform Si6, OTTHUMP00000196197, desmocollin type 4
Official Symbol: BID
Accession Number(s): NP_932070.1; NP_001187.1; NP_932071.1
Human GeneID(s): 637
Important Comments: This antibody is expected to recognise all three reported isoforms (as represented by NP_932070.1; NP_001187.1; NP_932071.1)

Immunogen

Peptide with sequence C-TYVRSLARNGMD, from the C Terminus of the protein sequence according to NP_932070.1; NP_001187.1; NP_932071.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:8000.
Western blot: Approx 26+24kDa bands observed in A431 cell lysates (calculated MW of 26.8kDa according to NP_932070.1 and 22.0kDa according to NP_001187.1). Recommended concentration: 0.1-0.3µg/ml. Primary incubation 1 hour at room temperature.

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of MCF7 cells. Recommended concentration: 10µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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