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Goat Anti-Biglycan Preproprotein Antibody (EB07505)

Code Name Applications Availability Size Tested species Price Grade  
EB07505 Goat Anti-Biglycan Preproprotein Antibody Pep-ELISA, IF Yes 100µg specific antibody in 200µl Human, Mouse $324.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: info@arp1.com
www.arp1.com

EB07505 1
EB07505 Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing Golgi staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB07505 2
EB07505 Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing Golgi/ER staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Click here to download PDF datasheet.

Target Protein

Principal Names: Biglycan Preproprotein, biglycan, BGN, DSPG1, PG-S1, PGI, SLRR1A, bone/cartilage proteoglycan-I, dermatan sulphate proteoglycan I, small leucine-rich protein 1A
Official Symbol: BGN
Accession Number(s): NP_001702.1
Human GeneID(s): 633
Non-Human GeneID(s): 12111 (mouse), 25181 (rat)

Immunogen

Peptide with sequence C-DRLAIQFGNYKK, from the C Terminus of the protein sequence according to NP_001702.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:2000.

Immunofluorescence: Expression of the protein seen in the Golgi of NIH3T3 and HepG2 cells. Recommended concentration: 10µg/ml.

Species Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Rat, Dog

Specific Reference

This antibody has been successfully used in IHC on Human:
Burssens A, Forsyth R, Bongaerts W, Jagodzinski M, Mahieu N, Praet M, Victor J.
Arguments for an increasing differentiation towards fibrocartilaginous components in midportion Achilles tendinopathy.
Knee Surg Sports Traumatol Arthrosc. 2012 Sep 23.
PMID: 23001016

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