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Goat Anti-BLNK / SLP-65 Antibody - Trial (EB05111-T)

Related Products: EBP05111 - Immunizing Peptide, Goat Anti-BLNK / SLP-65 Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB05111-T Goat Anti-BLNK / SLP-65 Antibody - Trial Pep-ELISA, WB, IF, FC Yes 20µg specific antibody in 40µl Human $105.00 Elite_Plus

Ordering Information for

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$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
TestShot
EB05111-T 1
EB05111 (0.3µg/ml) staining of Daudi cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB05111-T 2
EB05111 Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing some membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB05111-T 3
EB05111 Flow cytometric analysis of paraformaldehyde fixed Daudi cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: BLNK, SLP-65, B-cell linker, BASH, BLNK-s, Ly57, MGC111051, SLP65, B cell linker protein, B-cell adapter containing a SH2 domain protein, B-cell adapter containing a Src homology 2 domain protein, OTTHUMP00000020167, Src homology 2 domain-containing leukocyte protein of 65 kDa, BLNK-S, LY57, OTTHUMP00000020168
Official Symbol: BLNK
Accession Number(s): NP_037446.1; NP_001107566.1; NP_001245369.1; NP_001245370.1; NP_001245371.1
Human GeneID(s): 29760
Non-Human GeneID(s): 17060 (mouse)
Important Comments: This antibody is expected to recognize all reported isoforms.

Immunogen

Peptide with sequence C-KDSTRLKYAVKVS, from the C Terminus of the protein sequence according to NP_037446.1; NP_001107566.1; NP_001245369.1; NP_001245370.1; NP_001245371.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 70-75kDa band observed in lysates of cell line Daudi (calculated MW of 50.5kDa according to NP_037446.1). This molecular weight is routinely observed by other sources. Recommended concentration 0.3-1µg/ml. Primary incubation 1 hour at room temperature.

Immunofluorescence: Strong expression of the protein seen in the membranes of HepG2 cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of Daudi cells. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

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