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Goat Anti-BMI1 (aa237-251) Antibody (EB12495)

Related Products: EBP12495 - Immunizing Peptide, EBP12496 - Immunizing Peptide, Goat Anti-BMI1 Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB12495 Goat Anti-BMI1 (aa237-251) Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human, Mouse, Pig $411.00 Elite

Ordering Information for

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our US partner Abcore LLC.

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Now shipping direct to US customers! 99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]), fax (888-841-9041), phone (888-320-4628). Please include the following information with your order:

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In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day.

Orders are shipped by our US logistics partner Abcore LLC.

 

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Order from our local distributor

ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB12495 1
EB12495 (0.1µg/ml) staining of K562 nuclear lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB12495 2
EB12495 (0.3µg/ml) staining of NIH3T3 nuclear lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB12495 3
EB12495 (2µg/ml) staining of Pig Bone Marrow lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB12495 4
EB12495 (5µg/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: BMI1, BMI1 polycomb ring finger oncogene, FLVI2/BMI1, PCGF4, RNF51, B lymphoma Mo-MLV insertion region 1 homolog, flvi-2/bmi-1, murine leukemia viral (bmi-1) oncogene homolog, polycomb complex protein BMI-1, polycomb group RING finger protein 4, polycomb
Official Symbol: BMI1
Accession Number(s): NP_005171.4
Human GeneID(s): 648
Non-Human GeneID(s): 12151 (mouse)

Immunogen

Peptide with sequence C-SHQRDGLTNAGELES, from the internal region of the protein sequence according to NP_005171.4.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 40kDa band observed in nuclear lysates of cell lines K562 and NIH3T3 (calculated MW of 36.9kDa according to NP_005171.4). This band corresponds to earlier findings in literature with different antibodies (Chagraoui et al, Genes Dev. 2006 Aug 1;20(15):2110-20. PMID: 16882984). Recommended concentration: 0.1-0.3µg/ml. Approx 37kDa band observed in Pig Bone Marrow lysates (calculated MW of 36.9kDa according to NP_001272900.1 ). Recommended concentration: 1-3µg/ml.
IHC: Paraffin embedded Human Testis. Recommended concentration: 5µg/ml.

Species Reactivity

Tested: Human, Mouse, Pig
Expected from sequence similarity: Human, Mouse, Dog, Pig

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