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Goat Anti-BRG1 / SMARCA4 Antibody (EB06353)

Related Products: EBP06353 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB06353 Goat Anti-BRG1 / SMARCA4 Antibody Pep-ELISA, WB, IF, IHC, FC Yes 100µg specific antibody in 200µl Human $464.00 Elite_Plus

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EB06353 1
EB06353 (0.01ug/ml) staining of nuclear Jurkat cell lysate (A) + peptide (B). (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB06353 2
EB06353 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB06353 3
EB06353 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB06353 4
EB06353 Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
EB06353 5
EB06353 (3.75µg/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB06353 6
EB06353 (3.75µg/ml) staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB06353 7
EB06353 (3.75µg/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: SMARCA4, BRG1, BAF190, SNF2L4, SNF2LB, hSNF2B, hSNF2b, SNF2-BETA, SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4, SNF2-beta, SNF2-like 4, nuclear protein GRB1, brahma protein-like 1, BRM/SWI2-related gene 1, homeotic gene regulator, sucrose nonfermenting-like 4, mitotic growth and transcription activator, global transcription activator homologous sequence
Official Symbol: SMARCA4
Accession Number(s): NP_001122321.1; NP_003063.2; NP_001122317.1; NP_001122318.1; NP_001122319.1; NP_001122320.1
Human GeneID(s): 6597
Non-Human GeneID(s): 20586 (mouse), 171379 (rat)
Important Comments: This antibody is expected to recognize all reported isoforms (NP_001122321.1; NP_003063.2; NP_001122317.1; NP_001122318.1; NP_001122319.1; NP_001122320.1). Reported variants NP_003063.2 and NP_001122316.1 represent identical protein.

Immunogen

Peptide with sequence STPDPPLGGTPRP-C, from the N Terminus of the protein sequence according to NP_001122321.1; NP_003063.2; NP_001122317.1; NP_001122318.1; NP_001122319.1; NP_001122320.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx. 200-250kDa band observed in nuclear lysates of cell line Jurkat (calculated MW of 185kDa according to NP_003063.2). This molecular weight is observed by other commercial sources, and was blocked by incubation with the immunizing peptide. Recommended concentration: 0.01-0.03ug/ml. Primary incubation 1 hour at room temperature.
IHC: Paraffin embedded Human Small Intestine, Tonsil and Prostate. Recommended concentration: 3.75µg/ml.

Immunofluorescence: Strong expression of the protein seen in the nuclei of A431 and U2OS cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog

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