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Goat Anti-BTRC / FWD1 Antibody (EB06199)

Related Products: EBP06199 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB06199 Goat Anti-BTRC / FWD1 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB06199 1
EB06199 (1µg/ml) staining of Nuclear lysates of cell line HeLa (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB06199 2
EB06199 (7.5µg/ml) staining of paraffin embedded Human Heart. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
EB06199 3
EB06199 Negative Control showing staining of paraffin embedded Human Heart, with no primary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: BTRC, beta-transducin repeat containing, RP11-529I10.2, BETA-TRCP, FBW1A, FBXW1, FBXW1A, FWD1, MGC4643, bTrCP, bTrCP1, betaTrCP, E3RSIkappaB, F-box and WD repeats protein beta-TrCP, F-box and WD-repeat protein 1B, F-box/WD repeat-containing protein 1A, OTTHUMP00000020314, OTTHUMP00000020315, beta-TrCP1, pIkappaBalpha-E3 receptor subunit
Official Symbol: BTRC
Accession Number(s): NP_378663.1; NP_003930.1; NP_001243785.1
Human GeneID(s): 8945
Non-Human GeneID(s): 12234 (mouse), 361765 (rat)
Important Comments: This antibody is expected to recognise both reported isoforms (NP_378663.1; NP_003930.1).

Immunogen

Peptide with sequence DPAEAVLQEKALK-C, from the N Terminus of the protein sequence according to NP_378663.1; NP_003930.1; NP_001243785.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 65kDa band observed in nuclear lysates of cell line HeLa (calculated MW of 65kDa according to NP_003930.1 and 68.5kDa according to NP_001243785.1. Recommended concentration: 1-3µg/ml. Primary incubation was 1 hour.
IHC: Paraffin embedded Human Heart. Recommended concentration: 7.5µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Pig, Cow

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