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Goat Anti-C14orf68 (aa242-254) Antibody (EB07605)

Related Products: EBP07604 - Immunizing Peptide, EBP07605 - Immunizing Peptide, Goat Anti-C14orf68 Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB07605 Goat Anti-C14orf68 (aa242-254) Antibody Pep-ELISA, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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ARP American Research Products, Inc
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EB07605 1
EB07605 (2.5µg/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: C14orf68, chromosome 14 open reading frame 68, HDMCP, HMFN1655, hepatocellular carcinoma-downregulated mitochondrial carrier protein
Official Symbol: SLC25A47
Accession Number(s): NP_997000.2
Human GeneID(s): 283600

Immunogen

Peptide with sequence C-KSRLQADGQGQRR, from the internal region of the protein sequence according to NP_997000.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Preliminary experiments gave an approx 22kDa band in Human Liver lysates after 0.5µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 33.4kDa according to NP_997000.2. The 22kDa band was successfully blocked by incubation with the immunizing peptide.
IHC: In paraffin embedded Human Liver shows staining of nucleoli in select hepatocytes. Recommended concentration, 2.5µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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